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download endless ocean blue world onlineIdentification of a population of vascular progenitor cells in murine aortas.
Aortic endothelial cells are repaired and functionally integrated within the aortic wall via the processes of intimal hyperplasia and neointima formation. While the vascular progenitor cells that participate in these processes have been identified in other vascular beds, the existence of these cells in aortic endothelium remains to be established. We developed a mouse model in which aortic endothelial cells are ablatively ablated, thus allowing examination of the characteristics of these cells as well as their ability to form new endothelium. Aortae from mice between 4 and 8 weeks of age were removed and the endothelium removed. The aortic ring-containing media and adventitia were removed from the vessel and cultured in collagen-Matrigel to allow for cell growth. Aortic vessels from 4-week-old mice displayed morphologically distinct colonies of cells that were round in shape and in contact with the growth surface. These cells were localized to the medial layer of the vessel and were strongly positive for the cell surface markers CD34 and CD31. To investigate the phenotype of these cells further, aortic cells from mice between 8 and 12 weeks of age were cultured. These cells expressed CD31 (an endothelial cell marker) and CD90 (another endothelial cell marker) but not CD45 (a lymphocyte marker) or CD44 (a stem cell marker). A subpopulation of aortic cells expressing CD31, CD90, and CD45 was also identified. These cells were elongated in shape and were not attached to the growth surface of the Matrigel. In the presence of basic fibroblast growth factor, these cells also formed round colonies with cobblestone morphology. Collectively, the data suggest that vascular progenitor cells within the mouse aorta have the capacity to proliferate in culture and to organize into colony-forming cell clusters.This feature is being provided by the New Mexico Department of Workforce Solutions (DWS). If you would like to report a problem with the Unemployment Insurance (UI) Data Retrieval System, or if you have any questions regarding this feature, please contact us at (505) 582-6085. All complaints will be documented and addressed. 0b46394aab
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